goscripttm qpcr master mix Search Results


goscripttm qpcr master mix kit  (Promega)
90
Promega goscripttm qpcr master mix kit
Goscripttm Qpcr Master Mix Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+qpcr+master+mix/pm37454119-105-7-12?v=Promega
Average 90 stars, based on 1 article reviews
goscripttm qpcr master mix kit - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
goscripttm qpcr master mix  (Promega)
90
Promega goscripttm qpcr master mix
CircSLC39A5 is localized in the nucleus, binds to signal transducer and activator of transcription 1 (STAT1), and affects its expression and stability. (A, B) FISH and cytoplasmic separation experiments showed that circSLC39A5 was mainly localized in the nucleus of MHCC97H. (C–E) RNA pulldown, silver staining, and Western blot (WB) showed that circSLC39A5 could bind to STAT1 in Huh7. (F) The secondary structure (minimum free energy, MFE) of circSLC39A5 predicted by the RNAfold WebServer. (G) Graphical exhibition of molecular binding between circSLC39A5 and STAT1 protein (PDB ID: 1BF5) using the HDOCK Server. (H) The NucleicNet Webserver was used to predict the binding preference sites of RNA components on STAT1. Red, cytosine; purple, uracil; blue, adenine; cyan, guanine; yellow, phosphate; green, ribose. (I, J) RNA immunoprecipitation (RIP) assay and WB confirmed the binding of STAT1 and circSLC39A5 in Huh7. (K–M) <t>CLIP‐qPCR</t> and WB were used to verify the binding region of circSLC39A5 to STAT1. Eight primers were designed to cover the full‐length sequence of circSLC39A5, in which the corresponding sequence of primer 1 was across the splicing site. (N) The protein expression of STAT1 in Huh7 was detected by WB. (O) WB was used to detect the effect of MG132 treatment on STAT1 expression in Huh7. * p < 0.05, ** p < 0.01.
Goscripttm Qpcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+qpcr+master+mix/pmc10551608-77-21-25?v=Promega
Average 90 stars, based on 1 article reviews
goscripttm qpcr master mix - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
goscripttm rt mix for 1-step rt-qpcr  (Promega)
90
Promega goscripttm rt mix for 1-step rt-qpcr
CircSLC39A5 is localized in the nucleus, binds to signal transducer and activator of transcription 1 (STAT1), and affects its expression and stability. (A, B) FISH and cytoplasmic separation experiments showed that circSLC39A5 was mainly localized in the nucleus of MHCC97H. (C–E) RNA pulldown, silver staining, and Western blot (WB) showed that circSLC39A5 could bind to STAT1 in Huh7. (F) The secondary structure (minimum free energy, MFE) of circSLC39A5 predicted by the RNAfold WebServer. (G) Graphical exhibition of molecular binding between circSLC39A5 and STAT1 protein (PDB ID: 1BF5) using the HDOCK Server. (H) The NucleicNet Webserver was used to predict the binding preference sites of RNA components on STAT1. Red, cytosine; purple, uracil; blue, adenine; cyan, guanine; yellow, phosphate; green, ribose. (I, J) RNA immunoprecipitation (RIP) assay and WB confirmed the binding of STAT1 and circSLC39A5 in Huh7. (K–M) <t>CLIP‐qPCR</t> and WB were used to verify the binding region of circSLC39A5 to STAT1. Eight primers were designed to cover the full‐length sequence of circSLC39A5, in which the corresponding sequence of primer 1 was across the splicing site. (N) The protein expression of STAT1 in Huh7 was detected by WB. (O) WB was used to detect the effect of MG132 treatment on STAT1 expression in Huh7. * p < 0.05, ** p < 0.01.
Goscripttm Rt Mix For 1 Step Rt Qpcr, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+qpcr+master+mix/pmc11312051-118-23-31?v=Promega
Average 90 stars, based on 1 article reviews
goscripttm rt mix for 1-step rt-qpcr - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
goscripttm reverse transcription mix  (Promega)
90
Promega goscripttm reverse transcription mix
CircSLC39A5 is localized in the nucleus, binds to signal transducer and activator of transcription 1 (STAT1), and affects its expression and stability. (A, B) FISH and cytoplasmic separation experiments showed that circSLC39A5 was mainly localized in the nucleus of MHCC97H. (C–E) RNA pulldown, silver staining, and Western blot (WB) showed that circSLC39A5 could bind to STAT1 in Huh7. (F) The secondary structure (minimum free energy, MFE) of circSLC39A5 predicted by the RNAfold WebServer. (G) Graphical exhibition of molecular binding between circSLC39A5 and STAT1 protein (PDB ID: 1BF5) using the HDOCK Server. (H) The NucleicNet Webserver was used to predict the binding preference sites of RNA components on STAT1. Red, cytosine; purple, uracil; blue, adenine; cyan, guanine; yellow, phosphate; green, ribose. (I, J) RNA immunoprecipitation (RIP) assay and WB confirmed the binding of STAT1 and circSLC39A5 in Huh7. (K–M) <t>CLIP‐qPCR</t> and WB were used to verify the binding region of circSLC39A5 to STAT1. Eight primers were designed to cover the full‐length sequence of circSLC39A5, in which the corresponding sequence of primer 1 was across the splicing site. (N) The protein expression of STAT1 in Huh7 was detected by WB. (O) WB was used to detect the effect of MG132 treatment on STAT1 expression in Huh7. * p < 0.05, ** p < 0.01.
Goscripttm Reverse Transcription Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+qpcr+master+mix/pm38977508-118-11-10?v=Promega
Average 90 stars, based on 1 article reviews
goscripttm reverse transcription mix - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

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CircSLC39A5 is localized in the nucleus, binds to signal transducer and activator of transcription 1 (STAT1), and affects its expression and stability. (A, B) FISH and cytoplasmic separation experiments showed that circSLC39A5 was mainly localized in the nucleus of MHCC97H. (C–E) RNA pulldown, silver staining, and Western blot (WB) showed that circSLC39A5 could bind to STAT1 in Huh7. (F) The secondary structure (minimum free energy, MFE) of circSLC39A5 predicted by the RNAfold WebServer. (G) Graphical exhibition of molecular binding between circSLC39A5 and STAT1 protein (PDB ID: 1BF5) using the HDOCK Server. (H) The NucleicNet Webserver was used to predict the binding preference sites of RNA components on STAT1. Red, cytosine; purple, uracil; blue, adenine; cyan, guanine; yellow, phosphate; green, ribose. (I, J) RNA immunoprecipitation (RIP) assay and WB confirmed the binding of STAT1 and circSLC39A5 in Huh7. (K–M) CLIP‐qPCR and WB were used to verify the binding region of circSLC39A5 to STAT1. Eight primers were designed to cover the full‐length sequence of circSLC39A5, in which the corresponding sequence of primer 1 was across the splicing site. (N) The protein expression of STAT1 in Huh7 was detected by WB. (O) WB was used to detect the effect of MG132 treatment on STAT1 expression in Huh7. * p < 0.05, ** p < 0.01.

Journal: Cancer Science

Article Title: Nucleus‐localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription

doi: 10.1111/cas.15906

Figure Lengend Snippet: CircSLC39A5 is localized in the nucleus, binds to signal transducer and activator of transcription 1 (STAT1), and affects its expression and stability. (A, B) FISH and cytoplasmic separation experiments showed that circSLC39A5 was mainly localized in the nucleus of MHCC97H. (C–E) RNA pulldown, silver staining, and Western blot (WB) showed that circSLC39A5 could bind to STAT1 in Huh7. (F) The secondary structure (minimum free energy, MFE) of circSLC39A5 predicted by the RNAfold WebServer. (G) Graphical exhibition of molecular binding between circSLC39A5 and STAT1 protein (PDB ID: 1BF5) using the HDOCK Server. (H) The NucleicNet Webserver was used to predict the binding preference sites of RNA components on STAT1. Red, cytosine; purple, uracil; blue, adenine; cyan, guanine; yellow, phosphate; green, ribose. (I, J) RNA immunoprecipitation (RIP) assay and WB confirmed the binding of STAT1 and circSLC39A5 in Huh7. (K–M) CLIP‐qPCR and WB were used to verify the binding region of circSLC39A5 to STAT1. Eight primers were designed to cover the full‐length sequence of circSLC39A5, in which the corresponding sequence of primer 1 was across the splicing site. (N) The protein expression of STAT1 in Huh7 was detected by WB. (O) WB was used to detect the effect of MG132 treatment on STAT1 expression in Huh7. * p < 0.05, ** p < 0.01.

Article Snippet: Then, complementary DNA (cDNA) was synthesized according to the instructions of the GoScriptTM Reverse Transcription System (Promega). qPCR was performed using GoScriptTM qPCR Master Mix (Promega) and QuantStudioTM7 (Q7) Flex Real‐Time PCR System.

Techniques: Expressing, Silver Staining, Western Blot, Binding Assay, RNA Immunoprecipitation, Sequencing

CircSLC39A5 affects the expression of signal transducer and activator of transcription 1 (STAT1) and its target gene thymine DNA glycosylase (TDG) and the expression of proliferating cell nuclear antigen (PCNA). (A) Six databases were used to predict the target genes of STAT1. (B) Pathway analysis of target genes of STAT1 by the Kyoto Encyclopedia of Genes and Genomes (KEGG). (C, D) ChIP‐qPCR showed that STAT1 binds to the promoter regions of TDG, SRRM3, and PPP1R7 in Huh7. (E) Western blot (WB) confirmed that STAT1 could affect the expression of TDG, which was regulated by circSLC39A5. (F) Expression of TDG mRNA in Huh7 was detected by qRT‐PCR. (G) The protein expressions of TDG and PCNA in Huh7 were detected by WB. (H) Immunohistochemistry (IHC) was used to detect the positive expression of TDG and PCNA in tumor sections of nude mice. * p < 0.05, ** p < 0.01.

Journal: Cancer Science

Article Title: Nucleus‐localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription

doi: 10.1111/cas.15906

Figure Lengend Snippet: CircSLC39A5 affects the expression of signal transducer and activator of transcription 1 (STAT1) and its target gene thymine DNA glycosylase (TDG) and the expression of proliferating cell nuclear antigen (PCNA). (A) Six databases were used to predict the target genes of STAT1. (B) Pathway analysis of target genes of STAT1 by the Kyoto Encyclopedia of Genes and Genomes (KEGG). (C, D) ChIP‐qPCR showed that STAT1 binds to the promoter regions of TDG, SRRM3, and PPP1R7 in Huh7. (E) Western blot (WB) confirmed that STAT1 could affect the expression of TDG, which was regulated by circSLC39A5. (F) Expression of TDG mRNA in Huh7 was detected by qRT‐PCR. (G) The protein expressions of TDG and PCNA in Huh7 were detected by WB. (H) Immunohistochemistry (IHC) was used to detect the positive expression of TDG and PCNA in tumor sections of nude mice. * p < 0.05, ** p < 0.01.

Article Snippet: Then, complementary DNA (cDNA) was synthesized according to the instructions of the GoScriptTM Reverse Transcription System (Promega). qPCR was performed using GoScriptTM qPCR Master Mix (Promega) and QuantStudioTM7 (Q7) Flex Real‐Time PCR System.

Techniques: Expressing, ChIP-qPCR, Western Blot, Quantitative RT-PCR, Immunohistochemistry